Considerations for three-dimensional image reconstruction from experimental data in coherent diffractive imaging.

ABSTRACT: Diffraction before destruction using X-ray free-electron lasers (XFELs) has the potential to determine radiation-damage-free structures without the need for crystallization. This article presents the three-dimensional reconstruction of the Melbournevirus from single-particle X-ray diffraction patterns collected at the LINAC Coherent Light Source (LCLS) as well as reconstructions from simulated data exploring the consequences of different kinds of experimental sources of noise. The reconstruction from experimental data suffers from a strong artifact in the center of the particle. This could be reproduced with simulated data by adding experimental background to the diffraction patterns. In those simulations, the relative density of the artifact increases linearly with background strength. Th

SUBMITTER: Lundholm IV 

PROVIDER: S-EPMC6126651 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature