Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains.

ABSTRACT: This protocol describes a method for measuring the metabolism in Drosophila melanogaster larval and adult brains. Quantifying metabolism in whole organs provides a tissue-level understanding of energy utilization that cannot be captured when analyzing primary cells and cell lines. While this analysis is ex vivo, it allows for the measurement from a number of specialized cells working together to perform a function in one tissue and more closely models the in vivo organ. Metabolic reprogramming has been observed in many neurological diseases, including neoplasia, and neurodegenerative diseases. This protocol was developed to assist the D. melanogaster community's investigation of metabolism in neurological disease models using a commercially available metabolic analyzer. Measuring metabolis

SUBMITTER: Neville KE 

PROVIDER: S-EPMC6126682 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature