Integrin α2β1 decelerates proliferation, but promotes survival and invasion of prostate cancer cells.

ABSTRACT: High expression level of integrin α2β1 is a hallmark of prostate cancer stem cell like cells. The role of this collagen receptor is controversial since it is down regulated in poorly differentiated carcinomas, but concomitantly proposed to promote metastasis. Here, we show that docetaxel resistant DU145 prostate cancer cells express high levels of α2β1 and that α2β1^High subpopulation of DU145 cells proliferates slower than the cells representing α2β1^Low subpopulation. To further study this initial observation we used Crispr/Cas9 technology to create an α2β1 negative DU145 cell line. Furthermore, we performed rescue experiment by transfecting α2 knockout cells with vector carrying α2 cDNA or with an empty vector for appropriate control. When these two cell lines were c

SUBMITTER: Ojalill M 

PROVIDER: S-EPMC6126696 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature