MiR-193b and miR-30c-1^* inhibit, whereas miR-576-5p enhances melanoma cell invasion in vitro.

ABSTRACT: In cancer cells, microRNAs (miRNAs) are often aberrantly expressed resulting in impaired mRNA translation. In this study we show that miR-193b and miR-30c-1^* inhibit, whereas miR-576-5p accelerates invasion of various human melanoma cell lines. Using Boyden chamber invasion assays the effect of selected miRNAs on the invasive capacity of various human melanoma cell lines was analyzed. Upon gene expression profiling performed on transfected A375 cells, CTGF, THBS1, STMN1, BCL9, RAC1 and MCL1 were identified as potential targets. For target validation, qPCR, Western blot analyses or luciferase reporter assays were applied. This study reveals opposed effects of miR-193b / miR-30c-1^* and miR-576-5p, respectively, on melanoma cell invasion and on expression of BCL9 and MCL

SUBMITTER: Kordaß T 

PROVIDER: S-EPMC6126698 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature