Rapid, field-deployable method for collecting and preserving plant metabolome for biochemical and functional characterization.

ABSTRACT: Study of plant metabolome is a growing field of science that catalogs vast biochemical and functional diversity of phytochemicals. However, collecting and storing samples of plant metabolome, sharing these samples across the scientific community and making them compatible with bioactivity assays presents significant challenges to the advancement of metabolome research. We have developed a RApid Metabolome Extraction and Storage (RAMES) technology that allows efficient, highly compact, field-deployable collection and storage of libraries of plant metabolome. RAMES technology combines rapid extraction with immobilization of extracts on glass microfiber filter discs. Two grams of plant tissue extracted in ethanol, using a specially adapted Dremel® rotary tool, produces 25-35 replicas of 10 mm

SUBMITTER: Skubel SA 

PROVIDER: S-EPMC6126852 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature