Frequent Monitoring of C-Peptide Levels in Newly Diagnosed Type 1 Subjects Using Dried Blood Spots Collected at Home.

ABSTRACT:

Objective

To evaluate an approach to measure β-cell function by frequent testing of C-peptide concentrations in dried blood spots (DBSs).

Patients

Thirty-two children, aged 7 to 17 years, with a recent diagnosis of type 1 diabetes.

Design

Mixed-meal tolerance test (MMTT) within 6 and again at 12 months after diagnosis, with paired venous and DBS C-peptide sampling at 0 and 90 minutes. Weekly DBS C-peptide before and after standardized breakfasts collected at home.

Results

DBS and plasma C-peptide levels (n = 115) correlated strongly (r = 0·91; P < 0.001). The Bland-Altman plot indicated good agreement. The median number of home-collected DBS cards per participant was 24 over a median of 6.9 months. Repeated DBS C-peptide levels varied considerably within and be

SUBMITTER: Willemsen RH 

PROVIDER: S-EPMC6126892 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature