Clinical Significance of PD-L1⁺ Exosomes in Plasma of Head and Neck Cancer Patients.

ABSTRACT: Purpose: The microenvironment of head and neck squamous cell carcinomas (HNSCC) is highly immunosuppressive. HNSCCs expressing elevated levels of PD-L1 have especially poor outcome. Exosomes that carry PD-L1 and suppress T-cell functions have been isolated from plasma of patients with HNSCC. The potential contributions of PD-L1⁺ exosomes to immune suppression and disease activity are evaluated.Experimental Design: Exosomes isolated from plasma of 40 HNSCC patients by size exclusion chromatography were captured on beads using anti-CD63 Abs, stained for PD-1 and PD-L1 and analyzed by flow cytometry. The percentages and mean fluorescence intensities (MFI) of PD-L1⁺ and PD-1⁺ exosome/bead complexes were correlated with the patients' clinicopatholo

SUBMITTER: Theodoraki MN 

PROVIDER: S-EPMC6126905 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature