Chick cranial neural crest cells use progressive polarity refinement, not contact inhibition of locomotion, to guide their migration.

ABSTRACT: To move directionally, cells can bias the generation of protrusions or select among randomly generated protrusions. Here we use 3D two-photon imaging of chick branchial arch 2 directed neural crest cells to probe how these mechanisms contribute to directed movement, whether a subset or the majority of cells polarize during movement, and how the different classes of protrusions relate to one another. We find that, in contrast to Xenopus, cells throughout the stream are morphologically polarized along the direction of overall stream movement and do not exhibit contact inhibition of locomotion. Instead chick neural crest cells display a progressive sharpening of the morphological polarity program. Neural crest cells have weak spatial biases in filopodia generation and lifetime. Local bursts o

SUBMITTER: Genuth MA 

PROVIDER: S-EPMC6126999 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature