Targeted enrichment outperforms other enrichment techniques and enables more multi-species RNA-Seq analyses.

ABSTRACT: Enrichment methodologies enable the analysis of minor members in multi-species transcriptomic data. We compared the standard enrichment of bacterial and eukaryotic mRNA to a targeted enrichment using an Agilent SureSelect (AgSS) capture for Brugia malayi, Aspergillus fumigatus, and the Wolbachia endosymbiont of B. malayi (wBm). Without introducing significant systematic bias, the AgSS quantitatively enriched samples, resulting in more reads mapping to the target organism. The AgSS-enriched libraries consistently had a positive linear correlation with their unenriched counterparts (r² = 0.559-0.867). Up to a 2,242-fold enrichment of RNA from the target organism was obtained following a power law (r² = 0.90), with the greatest fold enrichment achieved in samples with th

SUBMITTER: Chung M 

PROVIDER: S-EPMC6127098 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature