Structure of the human plasma membrane Ca²⁺-ATPase 1 in complex with its obligatory subunit neuroplastin.

ABSTRACT: Plasma membrane Ca²⁺-ATPases (PMCAs) are key regulators of global Ca²⁺ homeostasis and local intracellular Ca²⁺ dynamics. Recently, Neuroplastin (NPTN) and basigin were identified as previously unrecognized obligatory subunits of PMCAs that dramatically increase the efficiency of PMCA-mediated Ca²⁺ clearance. Here, we report the cryo-EM structure of human PMCA1 (hPMCA1) in complex with NPTN at a resolution of 4.1 Å for the overall structure and 3.9 Å for the transmembrane domain. The single transmembrane helix of NPTN interacts with the TM_8-9-linker and TM10 of hPMCA1. The subunits are required for the hPMCA1 functional activity. The NPTN-bound hPMCA1 closely resembles the E1-Mg²⁺ structure of endo(sarco)plasmic reticulum Ca

SUBMITTER: Gong D 

PROVIDER: S-EPMC6127144 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature