Whole Transcriptome Sequencing Analysis of the Synergistic Antimicrobial Effect of Metal Oxide Nanoparticles and Ajoene on Campylobacter jejuni.

ABSTRACT: Two metal oxide (i.e., Al₂O₃ and TiO₂) nanoparticles and ajoene, a garlic-derived organosulfur compound, were identified to be effective antimicrobials against Campylobacter jejuni, a leading cause of human gastrointestinal diseases worldwide. A significant synergistic antimicrobial effect was observed using ajoene and Al₂O₃/TiO₂ nanoparticles in a combined manner to cause at least 8 log₁₀ CFU/mL reduction of C. jejuni cells. Whole transcriptome sequencing (RNA-seq) and confocal micro-Raman spectroscopic analyses revealed the antimicrobial mechanism and identified the roles of ajoene and metal oxide nanoparticles in the synergistic treatment. Ajoene and metal oxide nanoparticles mediated a two-phase

SUBMITTER: Xue R 

PROVIDER: S-EPMC6127312 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature