Ligand-Induced Coupling between Oligomers of the M₂ Receptor and the G_i1 Protein in Live Cells.

ABSTRACT: Uncertainty over the mechanism of signaling via G protein-coupled receptors (GPCRs) relates in part to questions regarding their supramolecular structure. GPCRs and heterotrimeric G proteins are known to couple as monomers under various conditions. Many GPCRs form oligomers under many of the same conditions, however, and the biological role of those complexes is unclear. We have used dual-color fluorescence correlation spectroscopy to identify oligomers of the M₂ muscarinic receptor and of G_i1 in purified preparations and live Chinese hamster ovary cells. Measurements on differently tagged receptors (i.e., eGFP-M₂ and mCherry-M₂) and G proteins (i.e., eGFP-Gα_i1β₁γ₂ and mCherry-Gα_i1β₁γ_2</

SUBMITTER: Li Y 

PROVIDER: S-EPMC6127463 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature