Topical Lyophilized Targeted Lipid Nanoparticles in the Restoration of Skin Barrier Function following Burn Wound.

ABSTRACT: Lyophilized keratinocyte-targeted nanocarriers (TLN_κ) loaded with locked nucleic acid (LNA) modified anti-miR were developed for topical application to full thickness burn injury. TLN_κ were designed to selectively deliver LNA-anti-miR-107 to keratinocytes using the peptide sequence ASKAIQVFLLAG. TLN_κ employed DOTAP/DODAP combination pH-responsive lipid components to improve endosomal escape. To minimize interference of clearance by non-targeted cells, especially immune cells in the acute wound microenvironment, surface charge was neutralized. Lyophilization was performed to extend the shelf life of the lipid nanoparticles (LNPs). Encapsulation efficiency of anti-miR in lyophilized TLN_κ was estimated to be 96.54%. Cargo stability of lyophilized TL

SUBMITTER: Li J 

PROVIDER: S-EPMC6127501 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature