Hyphenation of Supercritical Fluid Chromatography and NMR with In-Line Sample Concentration.

ABSTRACT: By coupling supercritical fluid chromatography (SFC) and nuclear magnetic resonance (NMR) in-line, a powerful analytical method arises that enables chemically specific analysis of a broad range of complex mixtures. However, during chromatography, the compounds are diluted in the mobile phase, in this case supercritical CO₂ (scCO₂), often resulting in concentrations that are too low to be detected by NMR spectroscopy or at least requiring excessive signal averaging. We present a hyphenated SFC-NMR setup with an integrated approach for concentrating samples in-line, which are diluted in scCO₂ during chromatography. This in-line concentration is achieved by controlled in-line expansion of the scCO₂. As a proof of concept four isomers of vitamin E (t

SUBMITTER: van Zelst FHM 

PROVIDER: S-EPMC6127797 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature