Spatial Proliferation of Epithelial Cells Is Regulated by E-Cadherin Force.

ABSTRACT: Cell proliferation and contact inhibition play a major role in maintaining epithelial cell homeostasis. Prior experiments have shown that externally applied forces, such as stretch, result in increased proliferation in an E-cadherin force-dependent manner. In this study, the spatial regulation of cell proliferation in large epithelial colonies was examined. Surprisingly, cells at the center of the colony still had increased proliferation as compared to cells in confluent monolayers. E-cadherin forces were found to be elevated for both cells at the edge and center of these larger colonies when compared to confluent monolayers. To determine if high levels of E-cadherin force were necessary to induce proliferation at the center of the colony, a lower-force mutant of E-cadherin was developed.

SUBMITTER: Mohan A 

PROVIDER: S-EPMC6127877 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature