Reliability of a participant-friendly fecal collection method for microbiome analyses: a step towards large sample size investigation.

ABSTRACT:

Background

The effects of gut microbiota on human traits are expected to be small to moderate and adding the complexity of the human diseases, microbiome research demands big sample sizes. Fecal samples for such studies are mostly self-collected by participants at home. This imposes an extra level of complexity as sample collection and storage can be challenging. Effective, low-burden collection and storage methods allowing fecal samples to be transported properly and ensuring optimal quality and quantity of bacterial DNA for upstream analyses are necessary. Moreover, accurate assessment of the microbiome composition also depends on bacterial DNA extraction method. The aim of this study was to evaluate the reliability and efficiency of the OMNIgene•GUT kit as a participant-fecal fr

SUBMITTER: Szopinska JW 

PROVIDER: S-EPMC6127955 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature