Phosphatidylinositol transfer proteins regulate megakaryocyte TGF-β1 secretion and hematopoiesis in mice.

ABSTRACT: We hypothesized that megakaryocyte (MK) phosphoinositide signaling mediated by phosphatidylinositol transfer proteins (PITPs) contributes to hematopoietic stem cell (HSC) and hematopoietic progenitor cell (HPC) regulation. Conditional knockout mice lacking PITPs specifically in MKs and platelets (pitpα^-/- and pitpα^-/-/β^-/-) bone marrow (BM) manifested decreased numbers of HSCs, MK-erythrocyte progenitors, and cycling HPCs. Further, pitpα^-/-/β^-/- BM had significantly reduced engrafting capability in competitive transplantation and limiting dilution analysis. Conditioned media (CM) from cultured pitpα^-/- and pitpα^-/-/β^-/- BM MKs contained higher levels of t

SUBMITTER: Capitano M 

PROVIDER: S-EPMC6128088 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature