SRPK1 is a poor prognostic indicator and a novel potential therapeutic target for human colorectal cancer.

ABSTRACT:

Background

Serine/arginine protein kinase 1 (SRPK1) is a protein kinase that belongs to the serine/arginine-rich domain family of splicing factors which are essential for splice-site selection, especially the modulation for RNA metabolism, localization, and translation. High expression of SRPK1 has been found in numerous human cancers, but its mechanism in colorectal cancer (CRC) is still rarely reported.

Purpose

To investigate the expression of SRPK1 in CRC tissues and cells and determine its functions and mechanism in CRC.

Methods

The expression of SRPK1 was explored in human CRC patients and cells by immunohistochemistry, real-time quantitative PCR, and Western blot; Cell Counting Kit-8, Transwell, flow cytometry, and tube formation assay were used to investigate t

SUBMITTER: Yi N 

PROVIDER: S-EPMC6128266 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature