Phospholipid synthesis fueled by lipid droplets drives the structural development of poliovirus replication organelles.

ABSTRACT: Rapid development of complex membranous replication structures is a hallmark of picornavirus infections. However, neither the mechanisms underlying such dramatic reorganization of the cellular membrane architecture, nor the specific role of these membranes in the viral life cycle are sufficiently understood. Here we demonstrate that the cellular enzyme CCTα, responsible for the rate-limiting step in phosphatidylcholine synthesis, translocates from the nuclei to the cytoplasm upon infection and associates with the replication membranes, resulting in the rerouting of lipid synthesis from predominantly neutral lipids to phospholipids. The bulk supply of long chain fatty acids necessary to support the activated phospholipid synthesis in infected cells is provided by the hydrolysis of neutral l

SUBMITTER: Viktorova EG 

PROVIDER: S-EPMC6128640 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature