Mechanism(s) of action of heavy metals to investigate the regulation of plastidic glucose-6-phosphate dehydrogenase.

ABSTRACT: The regulation of recombinant plastidic glucose-6P dehydrogenase from Populus trichocarpa (PtP2-G6PDH - EC 1.1.1.49) was investigated by exposing wild type and mutagenized isoforms to heavy metals. Nickel and Cadmium caused a marked decrease in PtP2-G6PDH WT activity, suggesting their poisoning effect on plant enzymes; Lead (Pb⁺⁺) was substantially ineffective. Copper (Cu⁺⁺) and Zinc (Zn⁺⁺) exposition resulted in strongest decrease in enzyme activity, thus suggesting a physiological competition with Magnesium, a well-known activator of G6PDH activity. Kinetic analyses confirmed a competitive inhibition by Copper, and a mixed inhibition by (Cd⁺⁺). Mutagenized enzymes were differently affected by HMs: the reduction of disulfide (C¹⁷⁵-C

SUBMITTER: De Lillo A 

PROVIDER: S-EPMC6128849 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature