Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy.

ABSTRACT: We present a correlative microscopy approach for biology based on holographic X-ray imaging, X-ray scanning diffraction, and stimulated emission depletion (STED) microscopy. All modalities are combined into the same synchrotron endstation. In this way, labeled and unlabeled structures in cells are visualized in a complementary manner. We map out the fluorescently labeled actin cytoskeleton in heart tissue cells and superimpose the data with phase maps from X-ray holography. Furthermore, an array of local far-field diffraction patterns is recorded in the regime of small-angle X-ray scattering (scanning SAXS), which can be interpreted in terms of biomolecular shape and spatial correlations of all contributing scattering constituents. We find that principal directions of anisotropic diffracti

SUBMITTER: Bernhardt M 

PROVIDER: S-EPMC6128893 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature