Characterization of a Listeria monocytogenes meningitis mouse model.

ABSTRACT:

Background

Listeria monocytogenes is a common cause of bacterial meningitis. We developed an animal model of listerial meningitis.

Methods

In survival studies, C57BL/6 mice received intracisternal injections with different L. monocytogenes sequence type 1 (ST1) colony forming units per milliliter (CFU; n = 48, 10⁵, 10⁶, 10⁷, 10⁸, and 10⁹ CFU/ml). Second, mice were inoculated with 10⁸ CFU/ml ST1 and sacrificed at 6 h and 24 h (n = 12/group). Outcome parameters were clinical score, CFUs, cyto- and chemokine levels, and brain histopathology. Third, 84 mice were inoculated (10⁹ CFU/ml ST1) to determine optimal antibiotic treatment with different doses of amoxicillin and gentamicin. Fourth, mice were ino

SUBMITTER: Koopmans MM 

PROVIDER: S-EPMC6128981 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature