The diversity of hydrogen-producing bacteria and methanogens within an in situ coal seam.

ABSTRACT:

Background

Biogenic and biogenic-thermogenic coalbed methane (CBM) are important energy reserves for unconventional natural gas. Thus, to investigate biogenic gas formation mechanisms, a series of fresh coal samples from several representative areas of China were analyzed to detect hydrogen-producing bacteria and methanogens in an in situ coal seam. Complete microbial DNA sequences were extracted from enrichment cultures grown on coal using the Miseq high-throughput sequencing technique to study the diversity of microbial communities. The species present and differences between the dominant hydrogen-producing bacteria and methanogens in the coal seam are then considered based on environmental factors.

Results

Sequences in the Archaea domain were classified into four phyla an

SUBMITTER: Su X 

PROVIDER: S-EPMC6128992 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature