Omega 3 polyunsaturated fatty acids inhibit cell proliferation by regulating cell cycle in fad3b transgenic mouse embryonic stem cells.

ABSTRACT:

Background

The consumption of omega 3 polyunsaturated fatty acids (PUFAs) is important for human health and is closely associated with cell proliferation and differentiation. This study aimed to investigate the influence of omega 3 PUFAs on embryonic stem cell (ESC) proliferation and explore potential mechanisms that mediate these effects.

Methods

In this study, we isolated ESCs from fad3b-expressing transgenic mice. We detected the fatty-acid composition of ESCs using gas chromatography-mass spectroscopy, analyzed cell-cycle phases using flow cytometry, and detected gene expression using real-time polymerase chain reaction (PCR) and western blots.

Results

The amount of omega 3 PUFAs significantly increased in fad3b versus control ESCs. However, the growth of fad3b ES

SUBMITTER: Wei Z 

PROVIDER: S-EPMC6129006 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature