Multivalent Cation-Bridged PI(4,5)P₂ Clusters Form at Very Low Concentrations.

ABSTRACT: Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂ or PIP2), is a key component of the inner leaflet of the plasma membrane in eukaryotic cells. In model membranes, PIP2 has been reported to form clusters, but whether these locally different conditions could give rise to distinct pools of unclustered and clustered PIP2 is unclear. By use of both fluorescence self-quenching and Förster resonance energy transfer assays, we have discovered that PIP2 self-associates at remarkably low concentrations starting below 0.05 mol% of total lipids. Formation of these clusters was dependent on physiological divalent metal ions, such as Ca²⁺, Mg²⁺, Zn²⁺, or trivalent ions Fe³⁺ and Al³⁺. Formation of PIP2 clusters was also headgroup-specific,

SUBMITTER: Wen Y 

PROVIDER: S-EPMC6129474 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature