A Nuclear Export Block Triggers the Decay of Newly Synthesized Polyadenylated RNA.

ABSTRACT: Genomes are promiscuously transcribed, necessitating mechanisms that facilitate the sorting of RNA for function or destruction. The polyA (pA) tail is one such distinguishing feature, which in the Saccharomyces cerevisiae nucleus is bound by the Nab2p protein, yielding transcript protection. As Nab2p also contacts the main nuclear export factor Mex67p, we asked whether transport kinetics contributes to RNA sorting. Indeed, 3' end sequencing of newly transcribed pA⁺ RNAs demonstrates that nuclear depletion of Mex67p elicits their instant and global decay. A similar phenotype is evident upon inactivation of other export factors and proportional to the amount of nuclear pA⁺ RNA. As RNA expression is partially rescued by Nab2p overexpression, we propose that an export blo

SUBMITTER: Tudek A 

PROVIDER: S-EPMC6130047 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature