Selective permeability of mouse blood-aqueous barrier as determined by ¹⁵N-heavy isotope tracing and mass spectrometry.

ABSTRACT: The blood-aqueous barrier plays a key role in regulating aqueous humor homeostasis by selectively restricting passage of proteins into the eye. The kinetics of aqueous flow are traditionally measured using artificial markers; however, these marker molecules do not address the barrier's selective permeability to plasma proteins. Here we applied stable isotope labeling of all serum proteins with nitrogen-15 (¹⁵N) atoms. Following systemic injection of this "heavy" serum in mice, the ¹⁵N-to-endogenous nitrogen-14 (¹⁴N) ratio of each protein in aqueous was measured by mass spectrometry. By monitoring the kinetic changes in these ratios, we determined the permeability profiles of hundreds of serum proteins. Meanwhile, we subjected one of the eyes to neoangiogeni

SUBMITTER: Liu P 

PROVIDER: S-EPMC6130361 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature