Convolutional neural networks automate detection for tracking of submicron-scale particles in 2D and 3D.

ABSTRACT: Particle tracking is a powerful biophysical tool that requires conversion of large video files into position time series, i.e., traces of the species of interest for data analysis. Current tracking methods, based on a limited set of input parameters to identify bright objects, are ill-equipped to handle the spectrum of spatiotemporal heterogeneity and poor signal-to-noise ratios typically presented by submicron species in complex biological environments. Extensive user involvement is frequently necessary to optimize and execute tracking methods, which is not only inefficient but introduces user bias. To develop a fully automated tracking method, we developed a convolutional neural network for particle localization from image data, comprising over 6,000 parameters, and used machine learning

SUBMITTER: Newby JM 

PROVIDER: S-EPMC6130393 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature