Isolation, characterization, and in silico, in vitro and in vivo antiulcer studies of isoimperatorin crystallized from Ostericum koreanum.

ABSTRACT:

Context

Ostericum koreanum (Maxim.) Kitagawa (Apiaceae) roots are traditionally used as an analgesic and antiulcer agent. However, the antiulcer potential of isoimperatorin isolated from O. koreanum has not yet been explored.

Aim

To evaluate the antiulcer activity of isoimperatorin isolated from the roots of O. koreanum.

Materials and methods

Isoimperatorin was isolated as cubic crystals by repeated column chromatography of the ethyl acetate fraction and structure was verified with ¹H NMR, ¹³C NMR and high-resolution mass spectrometry (HRMS-FAB). The crystals obtained were analyzed with the single crystal X-ray method. The MTT assay was used to determine its cytotoxicity against chondrocytes at different concentrations (0.0-737.74 μM, 24 h). The

SUBMITTER: Raza H 

PROVIDER: S-EPMC6130598 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature