Role of GABA_B receptors and p38MAPK/NF-κB pathway in paclitaxel-induced apoptosis of hippocampal neurons.

ABSTRACT:

Context

The effects of the anticancer drug paclitaxel on learning and memory are rarely studied.

Objective

This study investigated changes in GABA_B receptor expression during paclitaxel-induced apoptosis of hippocampal neurons and the role of the p38MAPK/NF-κB pathway in this process.

Materials and methods

Hippocampal neurons isolated from neonatal Sprague-Dawley rats were divided into six groups: Control (C), SB (10 µL of 10-µmol/L SB203580), SN (53 µg/mL SN50), N (1 µmol/L paclitaxel), SB + N (10 µmol/L SB203580 + 1 µmol/L paclitaxel) and SN + N (53 µg/mL SN50 + 1 µmol/L paclitaxel). Cells in different groups were treated with corresponding agents for 24 h at 37 °C. The apoptosis rate and protein levels of GABA_B1 receptors and NF-κB p65 were ev

SUBMITTER: Li Z 

PROVIDER: S-EPMC6130610 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature