Inhibition of IKKβ by celastrol and its analogues - an in silico and in vitro approach.

ABSTRACT:

Context

Alzheimer's disease (AD) is the most common form of dementia affecting the aged population and neuroinflammation is one of the most observed AD pathologies. NF-κB is the central regulator of inflammation and inhibitor κB kinase (IKK) is the converging point in NF-κB activation. Celastrol is a natural triterpene used as a treatment for inflammatory conditions.

Objective

This study determines the neuroprotective and inhibitory effect of celastrol on amyloid beta_1-42 (Aβ_1-42) induced cytotoxicity and IKKβ activity, respectively.

Materials and methods

Retinoic acid differentiated IMR-32 cells were treated with celastrol (1 μM) before treatment with Aβ_1-42 (IC₃₀ 10 μM) for 24 h. The cytotoxicity and IKK phosphorylation we

SUBMITTER: Veerappan K 

PROVIDER: S-EPMC6130723 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature