Fluorescence-based tools to probe G-quadruplexes in cell-free and cellular environments.

ABSTRACT: Biophysical and biochemical investigations provide compelling evidence connecting the four-stranded G-quadruplex (GQ) structure with its role in regulating multiple cellular processes. Hence, modulating the function of GQs by using small molecule binders is being actively pursued as a strategy to develop new chemotherapeutic agents. However, sequence diversity and structural polymorphism of GQs have posed immense challenges in terms of understanding what conformation a G-rich sequence adopts inside the cell and how to specifically target a GQ motif amidst several other GQ-forming sequences. In this context, here we review recent developments in the applications of biophysical tools that use fluorescence readout to probe the GQ structure and recognition in cell-free and cellular environment

SUBMITTER: Manna S 

PROVIDER: S-EPMC6130854 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature