Targeting light-gated chloride channels to neuronal somatodendritic domain reduces their excitatory effect in the axon.

ABSTRACT: Light-gated chloride channels are emerging as promising optogenetic tools for inhibition of neural activity. However, their effects depend on the transmembrane chloride electrochemical gradient and may be complex due to the heterogeneity of this gradient in different developmental stages, neuronal types, and subcellular compartments. Here we characterized a light-gated chloride channel, GtACR2, in mouse cortical neurons. We found that GtACR2 activation inhibited the soma, but unexpectedly depolarized the presynaptic terminals resulting in neurotransmitter release. Other light-gated chloride channels had similar effects. Reducing the chloride concentrations in the axon and presynaptic terminals diminished the GtACR2-induced neurotransmitter release, indicating an excitatory effect of chlori

SUBMITTER: Messier JE 

PROVIDER: S-EPMC6130974 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature