Radical solutions: Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure.

ABSTRACT: In recent years, electron capture (ECD) and electron transfer dissociation (ETD) have emerged as two of the most useful methods in mass spectrometry-based protein analysis, evidenced by a considerable and growing body of literature. In large part, the interest in these methods is due to their ability to induce backbone fragmentation with very little disruption of noncovalent interactions which allows inference of information regarding higher order structure from the observed fragmentation behavior. Here, we review the evolution of electron-based dissociation methods, and pay particular attention to their application in "native" mass spectrometry, their mechanism, determinants of fragmentation behavior, and recent developments in available instrumentation. Although we focus on the two most

SUBMITTER: Lermyte F 

PROVIDER: S-EPMC6131092 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature