A near complete, chromosome-scale assembly of the black raspberry (Rubus occidentalis) genome.

ABSTRACT:

Background

The fragmented nature of most draft plant genomes has hindered downstream gene discovery, trait mapping for breeding, and other functional genomics applications. There is a pressing need to improve or finish draft plant genome assemblies.

Findings

Here, we present a chromosome-scale assembly of the black raspberry genome using single-molecule real-time Pacific Biosciences sequencing and high-throughput chromatin conformation capture (Hi-C) genome scaffolding. The updated V3 assembly has a contig N50 of 5.1 Mb, representing an ∼200-fold improvement over the previous Illumina-based version. Each of the 235 contigs was anchored and oriented into seven chromosomes, correcting several major misassemblies. Black raspberry V3 contains 47 Mb of new sequences including lar

SUBMITTER: VanBuren R 

PROVIDER: S-EPMC6131213 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature