Protocols for Subtomogram Averaging of Membrane Proteins in the Dynamo Software Package.

ABSTRACT: Cryo-electron tomography allows low-resolution three-dimensional (3D) viewing of cellular organelles and macromolecular complexes present as multiple copies within a tomogram. These structures are computationally extracted and averaged in order to obtain high-resolution 3D structures, and provide a map of their spatial distribution and interaction with their biological microenvironment. To do so, we apply the user-friendly Dynamo software package on a tomographic data set. Dynamo acts as a modular toolbox adaptable to different biological scenarios, allowing a custom designed pipeline for subtomogram averaging. Here, we use as a textbook example the mitochondrial docking site of the positive-strand RNA Flock house nodavirus (FHV) to describe how Dynamo coordinates seve

SUBMITTER: Navarro PP 

PROVIDER: S-EPMC6131572 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature