Pre-Analytical Handling Conditions and Small RNA Recovery from Urine for miRNA Profiling.

ABSTRACT: There are currently no standardized protocols for pre-analytical handling of urine to best preserve small RNA for miRNA profiling studies. miRNA is an attractive candidate as a potential biomarker because of the high level of stability in body fluids and its ability to be quantified on multiple high-throughput platforms. We present a comparison of small RNA recovery and stability in urine under alternate pre-analytical handling conditions and extend recommendations on what conditions optimize yield of miRNA from cell-free urine and urine extracellular vesicles (EVs). Using an affinity slurry for isolation of small RNA from urine, we found that urine samples held at room temperature (20°C) for up to 8 hours before processing yield the highest amounts of intact small RNAs from EVs. Some miRN

SUBMITTER: Armstrong DA 

PROVIDER: S-EPMC6132192 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature