A qPCR method to quantify bioavailable phosphorus using indigenous aquatic species.

ABSTRACT:

Background

Bioavailable phosphorus (BAP) represents the sum of phosphorus that is readily available for algae growth and is useful to indicate the severity of eutrophication in aquatic environments.

Results

Here, a quantitative real-time PCR (qPCR)-based bioassay was developed to quantify BAP using the indigenous cyanobacterium species Anabaena sp. of Lake Tai, a large and shallow eutrophic lake in the Yangtze Valley, China. Primers were designed to quantify the gene expression of alkaline phosphatase (phoA/phoA-like) and phosphate transporter (pst1) genes of Anabaena. The specificity and efficiency of the primer sets were evaluated by gel electrophoresis and real-time PCR. The results showed that the primers developed here could suc

SUBMITTER: Yang Y 

PROVIDER: S-EPMC6132795 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature