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Mutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitis pigmentosa.

ABSTRACT: We identified a homozygous missense alteration (c.75C>A, p.D25E) in CLCC1, encoding a presumptive intracellular chloride channel highly expressed in the retina, associated with autosomal recessive retinitis pigmentosa (arRP) in eight consanguineous families of Pakistani descent. The p.D25E alteration decreased CLCC1 channel function accompanied by accumulation of mutant protein in granules within the ER lumen, while siRNA knockdown of CLCC1 mRNA induced apoptosis in cultured ARPE-19 cells. TALEN KO in zebrafish was lethal 11 days post fertilization. The depressed electroretinogram (ERG) cone response and cone spectral sensitivity of 5 dpf KO zebrafish and reduced eye size, retinal thickness, and expression of rod and cone opsins could be rescued by injection of wild type CLCC1 mRNA. Clcc1+/- KO mice showed decreased ERGs and photoreceptor number. Together these results strongly suggest that intracellular chloride transport by CLCC1 is a critical process in maintaining retinal integrity, and CLCC1 is crucial for survival and function of retinal cells.

SUBMITTER: Li L 

PROVIDER: S-EPMC6133373 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Mutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitis pigmentosa.

Li Lin L   Jiao Xiaodong X   D'Atri Ilaria I   Ono Fumihito F   Nelson Ralph R   Chan Chi-Chao CC   Nakaya Naoki N   Ma Zhiwei Z   Ma Yan Y   Cai Xiaoying X   Zhang Longhua L   Lin Siying S   Hameed Abdul A   Chioza Barry A BA   Hardy Holly H   Arno Gavin G   Hull Sarah S   Khan Muhammad Imran MI   Fasham James J   Harlalka Gaurav V GV   Michaelides Michel M   Moore Anthony T AT   Coban Akdemir Zeynep Hande ZH   Jhangiani Shalini S   Lupski James R JR   Cremers Frans P M FPM   Qamar Raheel R   Salman Ahmed A   Chilton John J   Self Jay J   Ayyagari Radha R   Kabir Firoz F   Naeem Muhammad Asif MA   Ali Muhammad M   Akram Javed J   Sieving Paul A PA   Riazuddin Sheikh S   Baple Emma L EL   Riazuddin S Amer SA   Crosby Andrew H AH   Hejtmancik J Fielding JF  

PLoS genetics 20180829 8

We identified a homozygous missense alteration (c.75C>A, p.D25E) in CLCC1, encoding a presumptive intracellular chloride channel highly expressed in the retina, associated with autosomal recessive retinitis pigmentosa (arRP) in eight consanguineous families of Pakistani descent. The p.D25E alteration decreased CLCC1 channel function accompanied by accumulation of mutant protein in granules within the ER lumen, while siRNA knockdown of CLCC1 mRNA induced apoptosis in cultured ARPE-19 cells. TALEN  ...[more]

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