Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry.

ABSTRACT: Desmosine (Des) and isodesmosine (Isodes), cross-linking amino acids in the biomolecule elastin, may be used as biomarkers for various pathological conditions associated with elastin degradation. The current study presents a novel approach to quantify Des and Isodes using matrix-assisted laser desorption ionization (MALDI)-tandem mass spectrometry (MS²) in a linear ion trap coupled to a vacuum MALDI source. MALDI-MS² analyses of Des and Isodes are performed using stable-isotope-labeled desmosine d₄ (labeled-Des) as an internal standard in different biological fluids, such as urine and serum. The method demonstrated linearity over two orders of magnitude with a detection limit of 0.02 ng/μL in both urine and serum without enrichment prior to mass spectrometr

SUBMITTER: Rathod P 

PROVIDER: S-EPMC6133720 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature