A β1-tubulin-based megakaryocyte maturation reporter system identifies novel drugs that promote platelet production.

ABSTRACT: During maturation, megakaryocytes (MKs) express β1-tubulin (TUBB1) and rearrange their microtubule components to enlarge, form proplatelets, and eventually release platelets. The development of a platform to identify in vitro conditions that would efficiently promote MK development could potentially enable large-scale platelet production. Here, we show that an immortalized MK cell line (imMKCL) genetically modified to express the β1-tubulin-Venus reporter provides a practical system to efficiently monitor the in vitro production of platelet-like particles (PLPs). The Venus transgene was inserted downstream of the TUBB1 locus in imMKCLs using CRISPR/Cas9, and the expression was visualized by Venus fluorescence intensity. This imMKCL reporter line was then used for high-throughput dru

SUBMITTER: Seo H 

PROVIDER: S-EPMC6134216 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature