Alterations in platelet secretion differentially affect thrombosis and hemostasis.

ABSTRACT: We genetically manipulated the major platelet vesicle-associated membrane proteins (VAMP2, VAMP3, and VAMP8) to create mice with varying degrees of disrupted platelet secretion. As previously shown, loss of VAMP8 reduced granule secretion, and this defect was exacerbated by further deletion of VAMP2 and VAMP3. VAMP2^Δ3^Δ8^-/- platelets also had reduced VAMP7. Loss of VAMP2 and VAMP3 (VAMP2^Δ3^Δ) had a minimal impact on secretion when VAMP7 and VAMP8 were present. Integrin αIIbβ3 activation and aggregation were not affected, although spreading was reduced in VAMP2^Δ3^Δ8^-/- platelets. Using these mice as tools, we asked how much secretion is needed for proper thrombosis and hemostasis in vivo. VAMP2^Δ3

SUBMITTER: Joshi S 

PROVIDER: S-EPMC6134224 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature