Optimization of the Linker Length of Mannose-Cholesterol Conjugates for Enhanced mRNA Delivery to Dendritic Cells by Liposomes.

ABSTRACT: Liposomes (LPs) as commonly used mRNA delivery systems remain to be rationally designed and optimized to ameliorate the antigen expression of mRNA vaccine in dendritic cells (DCs). In this study, we synthesized mannose-cholesterol conjugates (MP_n-CHs) by click reaction using different PEG units (PEG₁₀₀, PEG₁₀₀₀, and PEG₂₀₀₀) as linker molecules. MP_n-CHs were fully characterized and subsequently used to prepare DC-targeting liposomes (MP_n-LPs) by a thin-film dispersion method. MP_n-LPs loaded with mRNA (MP_n-LPX) were finally prepared by a simple self-assembly method. MP_n-LPX displayed bigger diameter (about 135 nm) and lower zeta potential (about 40 mV) compared to MP_n-LPs. The in

SUBMITTER: Wang F 

PROVIDER: S-EPMC6134263 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature