Searching for 3D structural models from a library of biological shapes using a few 2D experimental images.

ABSTRACT:

Background

Advancements in biophysical experimental techniques have pushed the limits in terms of the types of phenomena that can be characterized, the amount of data that can be produced and the resolution at which we can visualize them. Single particle techniques such as Electron Microscopy (EM) and X-ray free electron laser (XFEL) scattering require a large number of 2D images collected to resolve three-dimensional (3D) structures. In this study, we propose a quick strategy to retrieve potential 3D shapes, as low-resolution models, from a few 2D experimental images by searching a library of 2D projection images generated from existing 3D structures.

Results

We developed the protocol to assemble a non-redundant set of 3D shapes for generating the 2D image library, and to r

SUBMITTER: Tiwari SP 

PROVIDER: S-EPMC6134691 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature