Expression of alternative NADH dehydrogenases (NDH-2) in the phytopathogenic fungus Ustilago maydis.

ABSTRACT: Type 2 alternative NADH dehydrogenases (NDH-2) participate indirectly in the generation of the electrochemical proton gradient by transferring electrons from NADH and NADPH into the ubiquinone pool. Due to their structural simplicity, alternative NADH dehydrogenases have been proposed as useful tools for gene therapy of cells with defects in the respiratory complex I. In this work, we report the presence of three open reading frames, which correspond to NDH-2 genes in the genome of Ustilago maydis. These three genes were constitutively transcribed in cells cultured in YPD and minimal medium with glucose, ethanol, or lactate as carbon sources. Proteomic analysis showed that only two of the three NDH-2 were associated with isolated mitochondria in all culture media. Oxygen consumption

SUBMITTER: Matuz-Mares D 

PROVIDER: S-EPMC6134880 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature