Visualization of morphological categories of colonies for monitoring of effect on induced pluripotent stem cell culture status.

ABSTRACT: From the recent advances, there are growing expectations toward the mass production of induced pluripotent stem cells (iPSCs) for varieties of applications. For such type of industrial cell manufacturing, the technology which can stabilize the production efficiency is strongly required. Since the present iPSC culture is covered by delicate manual operations, there are still quality differences in produced cells from same culture protocols. To monitor the culture process of iPSCs with the quantified data to evaluate the culture status, we here introduce image-based visualization method of morphological diversity of iPSC colonies. We have set three types of experiments to evaluate the influential factors in iPSC culture technique that may disturb the undifferentiation status of iPSC colonies

SUBMITTER: Nagasaka R 

PROVIDER: S-EPMC6134894 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature