Matrix metalloproteinase (MMP) and immunosuppressive biomarker profiles of seven head and neck squamous cell carcinoma (HNSCC) cell lines.

ABSTRACT:

Background

Biomarkers like programmed death ligand-1 (PDL1) have become a focal point for immunotherapeutic checkpoint inhibition in head and neck squamous cell carcinoma (HNSCC). However, it's only part of the total immunosuppressive biomarker profile of HNSCC cells. Matrix metalloproteinases (MMPs) are enzymes that break down the basement membrane allowing cancer cells to metastasize and play an important role in the tumor microenvironment. MMPs can also activate certain cytokines, growth factors, and chemokines post-translationally. The objective of this study was to determine MMP and biomarker profiles of seven different HNSCC cell lines.

Methods

Authenticated cell lines were grown in minimal media at 1×10⁶ viable cells/mL and incubated at 37 °C. After 24 hrs

SUBMITTER: Bates AM 

PROVIDER: S-EPMC6135085 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature