Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda.

ABSTRACT: The intergenerational transfer of plant defense compounds by aposematic insects is well documented, and since 2006, has been shown for Cry toxins. Cry toxins are proteins naturally produced by the soil bacterium Bacillus thuringiensis (Bt) and its genes have been expressed in plants to confer insect pest resistance. In this work we tested if non-aposematic larvae of a major maize pest, Spodoptera frugiperda, with resistance to Cry1F, could transfer Cry1F from a genetically engineered maize variety to their offspring. Resistant 10-day-old larvae that fed on Cry1F Bt maize until pupation were sexed and pair-mated to produce eggs. Using ELISA we found that Cry1F was transferred to offspring (1.47-4.42 ng Cry1F/10 eggs), a toxin concentration about 28-83 times less than that detected in Cry1F

SUBMITTER: Souza CSF 

PROVIDER: S-EPMC6135484 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature