Can plant DNA barcoding be implemented in species-rich tropical regions? A perspective from Sao Paulo State, Brazil.

ABSTRACT: DNA barcoding helps to identify species, especially when identification is based on parts of organisms or life stages such as seeds, pollen, wood, roots or juveniles. However, the implementation of this approach strongly depends on the existence of complete reference libraries of DNA sequences. If such a library is incomplete, DNA-based identification will be inefficient. Here, we assess if DNA barcoding can already be implemented in species-rich tropical regions. We focus on the tree flora of São Paulo state, Brazil, which contains more than 2000 tree species. Using new DNA sequence data and carefully assembled GenBank accessions, we assembled 12,113 sequences from ten different regions. The ITS, rbcL, psbA-trnH, matK and trnL regions were better represented within the available sequences

SUBMITTER: Lima RAF 

PROVIDER: S-EPMC6136365 | biostudies-literature | 2018 Jul/Sept.

REPOSITORIES: biostudies-literature