Local Clonal Diversification and Dissemination of B Lymphocytes in the Human Bronchial Mucosa.

ABSTRACT: The efficacy of the adaptive humoral immune response likely requires diverse, yet focused regional B cell antibody production throughout the body. Here we address, in the first study of its kind, the B cell repertoire in the bronchial mucosa, an important barrier to antigens inhaled from the atmosphere. To accomplish this, we have applied high-throughput Adaptive Immune Receptor Repertoire Sequencing (AIRR-Seq) to 10 bronchial biopsies from altogether four different sites in the right lungs from an asthmatic patient and a healthy subject. While the majority of identified B cell clones were restricted to a single site, many were disseminated in multiple sites. Members of a clone were shared more between adjacent biopsies than between distal biopsies, suggesting local mucosal migration and/o

SUBMITTER: Ohm-Laursen L 

PROVIDER: S-EPMC6137163 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature